| Isolation of Protoplasts from Undaria pinnatifida by Alginate Lyase Digestion |
| HUXiaoke JIANGXiaolu GUANHuashi |
| 关键词：原生质体 藻酸 裂合酶 裂解酶 |
| 主要内容：The aim of this study is to isolate protoplasts from Undaria pinnatifida. Protoplasts of the alga were isolated enzymatically by using alginate lyase, which was prepared by fermenting culture of a strain Vibrio sp. 510. Monofacterial method was applied for optimizing digestion condition. The optimum condition for protoplast preparation is enzymatic digestionat 28℃ for 2 h using alginate lyase at the concentration of 213.36 U(8 mL) every 0.5g fresh thalline with Nacl 50 and at theshaking speed of 150rmin^-1 during digestion. The protoplast yield can reach 2.62±0.09 million per 0.5 g fresh leave under the optimum condition. The enzyme activity is inhibited by Ca^2+ and slightly enhanced by Fe^2+ and Mn^2+ at concentrations of 0.05, 0.08 and 0.10mol L^-1. |
| 《青岛海洋大学学报：英文版》 2003,2(1).-58-61 |
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