| HIGH DENSITY CULTIVATION OF A RECOMBINANT CD-1 CELL LINE PRODUCING PROUROKINASE USING A BIOSILON MICROCARRIER CULTURE SYSTEM |
| 肖成祖[1] 黄子才[1] 张正光[1] 叶建新[1] 高丽华[1] 郭智霞[1] 程度胜[1] 周鹤山[2] 孔惟惟[2] |
| 关键词：CD-1细胞链 高密度 细胞培养 培养基 Biosilon微载体培养系统 |
| 主要内容：CD-1,a genetically-engineered CHO cell line,was cultivated with a Biosilon microcarrier culture system.We successfully cultivated CD-1 cells to a very high density(over 1 ×10^7 cells/ml).Prourokinase was stably secreted at about 180IU/10^6 cells/24h.Experiments showed that CD-1 cells growing on Biosilon microcarriers were able to spontaneously release from the microcarriers,then reattach and proliferate on fresh microcarries.This makes it very easy to scale up production.The microcarries could be reused several times without affecting adhesion ,proliferation and prourokinase secretion.With CM-PECC membrane radial flow chromatography and MPG chromatography,the prouroknase in conditioned medium could be purfied to a specific activity of 1×10^5IU/mg of protein.The purification factor was about 600 fold,and approxiamately 90% of the biological activity was recovered. |
| 《中国医学科学杂志：英文版》 1994,9(4).-203-208 |
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