| Character of viral clones in serial passage of a nuclear polyhedrosis virus in vitro |
| LiGuoxun SongJie 等 |
| 关键词：核多角体病毒 传代 病毒克隆 感染力下降 |
| 主要内容：In this paper,the character of viral clones from early and late passages after serial passages of Trichoplusia ni single nuclear polyhedrosis virus in a Tn 5B1-4 cell line is described.It demonstrated that no significant difference was observed in the infectivity of the cell culture supernatants of various passages to the cell line.The number of polyhedra produced in a cell and infectivity of polyhedra to T.ni larvae declined strikingly with the increase of passages.The polyhedra without virions began to increase from passage to passage.The result of restriction enzyme digestion showed that the DNA restriction fragments of the clones were different from wild virus DNA,although they came from a homogeneous viral DNA.The mutation of viral DNA resulted in the in crease of noninfectious polyhedra without virions and in the increase of the number of polyhedra produced in cell line as well as virulence of the polyhydrosis inclusion bodys to T.ni larvae after prolonged passages of Tn SNPV in the cell culture. |
| 《东北农业大学学报：英文版》 1994,1(1).-25-32 |
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