| CLONING AND CHARACTERIZATION OF HUMAN HBIQUITIN BINDING ENZYME 2 cDNA |
| 李光涛[1] 吕鸿雁[2] |
| 关键词：人类泛素结合酶2 cDNA 基因克隆 鉴定 表达模式 蛋白降解 |
| 主要内容：Objective:To clone and identify the gene encoding human ubiquitin binding enzyme 2 and study is expression pattern.Methods:According to the sequence of human EST,which is highly homologous to the mouse ubiquitin binding/conjugating enzyme(E2),primers were synthesized to screen the human fetal brain cDNA library.The gene was analyzed by bioinformatics technique and is expression pattern was studied by using multiple-tissue Northern blot.Results:Two cDNA colnes encoding human ubiquitin conjugating enzyme have been isolated and identified.Both containing the ubiquitin coujugating domain,the 2 cDNA colnes are 88% identical in amino acid sequences and splicing isoforms to each other only with an exon excised to from the short sequence.They belong to a highly conserved and widely expressed E2 enzyme family.Northern blot shows that they are expressed exclusively in adult human heart,placenta,and pancreas but no transcripts can be detected in brain,lung ,liver,skeletal muscle or kidney.Conclusions:The gene encoding human ubiquitin binding enzyme is expressed under temporal control.As a key enzyme in the degradation of proteins,ubiquitin conjugating enzymes play a central role in the expression regulation on the level of post-translation. |
| 《中国医学科学杂志：英文版》 2002,17(1).-7-12 |
| 全文下载请进入http://hightech.stlib.cn/tpi_1/sysasp/include/index.asp |
微信公众号