| A human hepatoma cell line FLC4 cultured on the radial flow bioreactor as a model for human hepatocytes |
| LiYW[1] BabuE[2] |
| 关键词：FLC4细胞系 生物转化 药物代谢 肝细胞 |
| 主要内容：Hepatocytes play central roles in the metabolism and excretion of drugs and xenobiovics.For this purpose,hepatocytes were endowed with high levels of enzyme activity for the phase I and phase Ⅱ metabolism as well as high levels of transmembrane transport activity which enables the entrance and the exit of drugs and xenobiotics and their metabolites through the plasma membrane of the hepatocytes.They include the transporters in the canalicular and sinusoidal membrane.Although a lot of cell lines were established from hepatoma cells or normal hepatocytes,none of them are fully satisfactory in the expression of the enzymes and transportens.We have established and characterized a hepatoma cell line designated FLC4 and found that this cell line exhibits properties quite similar to those of the normal hepatocytes in the light of enzymes and transporters for drug metabolism and transkport when they are cultured on the radial flow bioreactors.Using FLC4 cells cultured on the radial flow bioreactors,we are developing in vitro systems to evaluate the interaction of drugs with liver transporters and drug-drug interaction through the hepa tocyte transporters. |
| 《中国药理学与毒理学杂志》 2002,16(6).-427-427 |
| 全文下载请进入http://hightech.stlib.cn/tpi_1/sysasp/include/index.asp |
微信公众号