| Detecting of PPNG by Molecuar Biology Technique |
| 谢平[1] 李琴[2] 张稷[2] 肖琛月[2] |
| 关键词：淋病 青霉素结合蛋白 PPNG基因 nPCR 流行病学 淋球菌 药物敏感性 耐药性 青霉素类药物 基因突变 |
| 主要内容：Objective: To investigate the prevalence of penicillinase-producing Neisseria gonorrhoeae (PPNG) in Wuxi. Methods: Nested PCR (nPCR) was used to test for the presence of β-Iactamase DNA in urethral or cervical swabs specimens from 217 patients with gonorrhea. One nPCR-positive product was randomly selected for DNA sequencing. Results: PPNG was found in 60 of all 217 specimens(27.65%), and in 33.87% (42/124) of male and 19.35%(18/93) of female cases. The nPCR product was identical to a known clinical PPNG strain. Conclusion: The prevalence of PPNG in STD patients in Wuxi is alarmingly high, and this must be taken into consideration for successful treatment and prevention of gonorrhea infections. |
| 《中华性传播感染杂志：英文版》 2002,2(2).-42-43 |
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