| Optimization of Media for Production of an Effective Yeast Biocontrol Agent Pichia membranefaciens |
| WANYa-kun TIANShi-ping |
| 关键词：酵母菌 生物防治 拮抗 小球藻生长因子 碳水化合物 |
| 主要内容：The growth of Pichia membranefaciens was studied using different nitrogen and carbon sources as substrates. Among nitrogen sources tested, soya peptone, yeast extract power, beef extract and polypeptone were relatively favorable to the growth of yeast. The density of the yeast showed to be directly proportional to carbon sources supplementation. Glucose and fructose were good carbon sources for the yeast growth. However, lactose showed poor performance for the cell growth of the yeast. In this study, beef extract presented a good synergic effect on the yeast growth with different carbonhydrates. The medium for P.membranefaciens used glucose and beef extract as substrates. The higher concentration of glucose and beef extract, the better growth of P.membranefaciens. The addition of chlorella growth factor (CGF) stimulated markedly the growth of P.membranefaciens. The increased concentration of CGF from 0.5 to 1% did not enhance the numbers of P.membranefaciens. This result will help design a better strategy for scale-up production of P.membranefaciens. |
| 《中国农业科学：英文版》 2004,3(4).-286-291 |
| 全文下载请进入http://hightech.stlib.cn/tpi_1/sysasp/include/index.asp |
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