| Cloning and Expression of Recombinant Human Thymosin in Yeast Pichia pastoris |
| 曹俊霞 JinLiji DuanYanlong AnLijia |
| 关键词：重组人胸腺激素α1基因 基因克隆 基因表达 酵母 PCR 核糖体蛋白 |
| 主要内容：The gene of human thyrnosin alpha 1 (hT(1)was synthesised according to favorite eodons of Pichia pastoris by PCR. N-terminal 28 amino acid residues of 40S ribosomal protein (RP), S24Ethat is N-aeetylserine were replaced by hT( 1 for the constitution of hT(1-RP fusion gene in order to express acetyllated thyrnosin α1. And also, the Asn-Gly bond was designed to faeiliate isolation of the target protein. The fusion gene was cloned into the expression vector, pPIC/gK. The constructs were transformed into HIS4 mutant strain GS115 by eleetroporation. Both SDS-PAGE analysis and Western blot analysis indicated that the fusion protein was expressed successfully. |
| 《高技术通讯：英文版》 2003,9(3).-60-64 |
| 全文下载请进入http://hightech.stlib.cn/tpi_1/sysasp/include/index.asp |
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