| Preparation of protein samples for gel electrophoresis by sequential extraction |
| 钟伯雄 翁宏飚 等 |
| 关键词：蛋白质 凝胶电泳 连续萃取 |
| 主要内容：Since preparation and solubilization of protein samples are crucial factors in proteome research,the authors established a sequential extraction technique to prepare protein samples from the body wall of the 5th instar larvae of silkworm.Bombyx mori.Two kinds of protein samples were obtained from the body wall using the method.Between the two types of samples only about 15% proteins were identical;the majority were different,indicating that more species of proteins could be obtained with the sequential extraction method;which will be useful for preparation of protein samples for proteome study. |
| 《浙江大学学报：自然科学英文版》 2002,3(5).-606-610 |
| 全文下载请进入http://hightech.stlib.cn/tpi_1/sysasp/include/index.asp |
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