| Analysis of the Protein Electrophoresis Patterns of Curvularia Lunata in Maize and Immunological Detection of the Pathogenic Culture |
| 宁红[1] QinZhen[2] 等 |
| 关键词：玉米 蛋白质 电泳图形 病原体培养 |
| 主要内容：Twelve isolates of Curvularia lunata and six related species from maize or other host plants are examined for the analysis of polyacrylamide gel electrophoresis (SDS-PAGE) patterns of their soluble proteins,Differences exist significantly among different species and the same species.There is a special protein band for Curvularia at relative mobility value 0.177(Rf 0.177) and a special protein band for C.lunata at Rf 0.225,Two kinds of rpimary antibodies to C.lunata are used to indirect ELISA and Avidin-Biotion-peroxidase Complex(ABC) immunoassay to evaluate their detecting sensitivity and specificity,The sensitivity of ABC immuno- assay is higher than indirect ELISA.APAbs to C.lunata is special to C.lunata. |
| 《高技术通讯：英文版》 2002,8(3).-11-15 |
| 全文下载请进入http://hightech.stlib.cn/tpi_1/sysasp/include/index.asp |
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