| A Co—expression System Based on Phage and Phagemid to Select Cognate Antibody—antigen Pairs in vivo |
| 胡学军 ZhangZhichao 等 |
| 关键词：生物过程 蛋白质－蛋白质相互作用 噬菌体 |
| 主要内容：A modified selectively-infective phage(SIP) is developed to facilitate the selection of interacting antibody-antigen paris from a large single-chan antibody(scFv)library in vivo.The system is constructed with a modified helper phage M13KO7 and phagemid pCANTAB 5E.The antigen fused to the C-terminal of N1-N2 domain and the scFv to the N-terminal of CT domain of the gIIIp of filamentous phage are encoded on the phage and phagemid vectors respectively.The phages produced by co-transformants restore infectivity via interaction between antigen and antibody fusions in the cell periplasm.In a model system,the scFv fragment of the anti-hemagglutinin 17/9 antibody and its corresponding antigen are detected in the presence of a 10^5 fold excess of a non-interacting cotrol paris,which demonstrates this system to be very sensitive and facile to screen a large single-chain antibody library. |
| 《高技术通讯：英文版》 2002,8(2).-5-9 |
| 全文下载请进入http://hightech.stlib.cn/tpi_1/sysasp/include/index.asp |
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