| Effects of wild-type （ Trp72 ） and mutant （ Arg72 ） apolipoprotein （a） kringle Ⅳ-10 on the proliferation of human arterial smooth muscle cells |
| 喻红[2] 洪嘉玲[1] 汪炳华[1] 彭芳芳[1] 李小明[1] 何春燕[1] |
| 关键词：动脉平滑肌细胞 细胞增殖 载脂蛋白a 转化生长因子β1 ELISA |
| 主要内容：Objective To assess the atherogenicity of lipoprotein(a), the effect of the heterogeneity of lysine binding of apolipoprotein (a) [ apo (a)], a plasminogen-like glycoprotein component on the proliferation of human arterial smooth muscle cells (SMCs).Methods Both wild type (wt) Trp72 and mutant (mut) Trp72→Arg forms of apo(a) kringle Ⅳ-10 were expressed by employing a GST-gene fusion system into E. coil The proliferation of SMCs was determined by flow cytometry and MTT colorimetry. Enzyme-linked immunosorbent assay (ELISA)assay was used to detect the active form of transforming growth factor β1 (TGF-β1).Results Apo(a) wt-kringle IV-10 that has lysine binding properties possessed a growth-stimulating activity to SMCs on a dose-dependence manner by stimulating cells in the G1/G0 phase of cell cycle to S and G2/M phase, and reduced significantly the amounts of endogenous active TGF-β1 in culture when compared with the control medium and the GST group (2.4 ± 0. 5 vs 8. 6 ± 1.6 and 9.1 ±1.7ng/ml, P<0.01 ). The growth-stimulating effect of apo(a) mut-kringle Ⅳ-10 deficient in lysine binding was negligible.Conclusions Apo(a) induces SMCs growth by inhibiting the activation of latent TGF-β1, an activitythat may involve the ability of apo(a) kringle Ⅳ-10 to bind lysine. The mitogenic effect of apo(a) wtkringle Ⅳ-10 on SMCs might play an active role in the atherogenic function of lipoprotein(a). |
| 《中华医学杂志：英文版》 2003,116(5).-721-726 |
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