| A Nficrocalorimetry and Spectroscopy Study on the Interaction of BSA with 2,2′-Bipyridine Octylglycinato Palladium（Ⅱ） Nitrate |
| MANSOORI-TORSHIZIHassan[1] ISLAMI-MOGHADDAMMahbobe[2] SABOURYAliAkbar[1] |
| 关键词：牛血清蛋白 钯复合物 相互作用 光谱学研究 |
| 主要内容：The interaction of bovine serum albumin (BSA) with a new palladium(Ⅱ) complex [ Pd(bpy) (Oct-Gly)] NO3 (bpy, 2,2′-bipyridine; Oct-Gly, octyl-glycine) was studied by isothermal titration UV-visible spectrophotometry and microcalorimetry in 30 mmol/L Tris buffer, pH 7.0. There is a set of 18 binding sites for this complex on BSA at 300 and 310 K with positive cooperativity in the binding process. The Hill coefficients at 300 and 310 K are 2.2 and 2.4, respectively. The binding of this palladium complex on BSA is endothermic with mean association binding constant of 21.0 and 16.4(mmol/L)^-1 at 300 and 310 K, respectively. The complex can denature the protein as surfactants. The stability of BSA in the interaction study with the complex is 84 and 58 kJ/mol at 300 and 310 K, respectively. Also, the enthalpy of BSA denaturation due to the interaction with the complex is 842 kJ/mol. |
| 《生物化学与生物物理学报：英文版》 2003,35(10).-886-890 |
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