| EXPRESSION AND LOCALIZATION OF SMAD4 PROTEIN IN RAT TESTIS DURING THE POSTNATAL DEVELOPMENT |
| 张远强[1] 胡静[1] 刘新平[2] 许若军[3] |
| 关键词：SMAD4蛋白 睾丸 转化生长因子-β 动物实验 |
| 主要内容：To study expression and localization of Smad4 protein, the common-mediator Smad, which is one of intracellular signaling molecules of transforming growth factor-β (TGF-β) family, in rat testis during postnatal development. Methods. In this study, whole testes were collected from S.D mrs aged 3 days, 7 days, 14 days and 28 days, and adult respectively. We examined the cellular localization and developmental change of Smad4 in rat testis by immunohistochemical ABC method with glucose oxidase-DAB-nickel enhancement technique; the quantitative analysis of the immunoataining by the image analysis system; the Smads proteins coexistence in the adult rat testis by the double immune staining for CD14-Smad4 and Smad2 -Smad4; the protein expression of Smed during rat testicular development by means of Western blots.Results. The protein of Smad4 was present in rats from 3 days of age to adulthood, and the immunolocalization was exclusively localized to the cytoplasm of Leydig ceils with negative nuclei in the interstitial tissue at any time point. No expression was detected in germ cells. The result of image and statistical analysis showed that generally, there was a tendency that the expression of Smad4 in the testes increased gradually with the rats developing maturation. Conclusion. Our data provide direct evidence for the molecular mechanism of TGF-β action in rat testes during postnatal development and spermatogenesis of rats. |
| 《中国医学科学杂志：英文版》 2003,18(3).-155-161 |
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