| Purification and characterization of Moschatin, a novel type I ribosomeinactivating protein from the mature seeds of pumpkin （Cucurbita moschata）, and preparation of its immunotoxin against human melanoma cells |
| FENGLI ZHENLI ZUCHUANZHANG HENGCHUANXA |
| 关键词：南瓜 种子 核糖体 免疫毒素 黑素瘤 |
| 主要内容：A novel ribosome-inactivating protein designated Moschatin from the mature seeds of pumpkin (Cucurbita moschata) has been successively purified to homogeneity, using ammonium sulfate precipitation, CM-cellulose 52 column chromatography, Blue Sepharose CL-6B Affinity column chromatography and FPLC size-exclusion column chromatography. Moschatin is a type 1 RIP with a pI of 9.4 and molecular weight of-29 kD. It is a rRNA Nglycosidase and potently blocked the protein synthesis in the rabbit reticulocyte lysate with a ICs0 of 0.26 nM. Using the anti-human melanoma McAb Ng76, a novel immunotoxin Moschatin-Ng76 was prepared successfully and it efficiently inhibited the growth of targeted melanoma cells M21 with a IC50 of 0.04 nM, 1500 times lower than that of free Moschatin. The results implied that Moschatin could be used as a new potential anticancer agent. |
| 《细胞研究：英文版》 2003,13(5).-369-374 |
| 全文下载请进入http://hightech.stlib.cn/tpi_1/sysasp/include/index.asp |
微信公众号