| Non－decoloured In－gel Digestion of Coomassie Blue－stained Proteins |
| ZHANGGuo-an BAOHui-min FANHui-zhi YANGPeng-yuan |
| 关键词：蛋白质 消化力 聚丙烯酰胺 凝胶 电泳 质谱分析 清蛋白 缩氨酸 |
| 主要内容：A simplified method is presented for tryptic digestion of Coomassie brilliant blue(CBB)-stained proteins in polyacrylamide gels. Compared with conventional methods, the proposed method does not require a re-moval of the dye before digestion, and is thus faster and saves a lot of labor. The resulted digest canbe ana-lyzed by either RPLC/ESIMS or MALDI MS for identification of the protein in a conventional way. Model studies with bovine serum albumin(BSA) showed that 50 ng of the protein could be routinely identified. The simplified procedure displays a tendency to produce more incompletely cleaved peptides, which is favorable for improving the sequence coverage. |
| 《高等学校化学研究：英文版》 2003,19(4).-399-403 |
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