| Constitutively Expressed αB—Crystallin in Heat Schock Transcription Factor 1 Knockout Mice Myocardium |
| 刘莉[1] 张洪慧[2] 丁国宪[2] 程蕴琳[2] 晏良军[2] BENJAMINIvorJ[2] |
| 关键词：小鼠 心肌层 热休克蛋白转录因子1 HSF1 αB－晶体蛋白 基因敲除 基因表达 |
| 主要内容：Objective:To invesligate the effects of heat shock transcription factor 1(HSF 1) gene on the constitutively expressed aB-Crystallin(αBC)in mice myocardium.Methods:The expression levels of constitutive αBC in HSF1 knockout(hsf1-/-) and HSF1 wild type (hsf1+/+) mice myocardium were evaluated by western blot and immunohistochemistry.Results:The αBC levels in hsf1-/- and hsf1+/_ were 68.42±4.16,100.00±7.58,respectively(P<0.05,cytosolic fraction),and 20.53±1.01,37.55±1.91,respectively(P<0.05,pellet fraction).The αBC signals decreased significantly in hsf1-/- myocardium when compared with those in hsf1+/+ myocardium stained with fluorescence immunohistochemistry.Conclusion.HSF1 is an important,but not the only factor,which mediates the constitutively expressed αBC. |
| 《南京医科大学学报：英文版》 2003,17(2).-67-73 |
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