| EST-derived microsatellites in Pseudosciaena crocea and their applicability to related species |
| YE Hua[1,2] WANG Xiaoqing[1] GAO Tianxiang[3] WANG Zhiyong[2] |
| 关键词：Pseudosciaena crocea,EST-SSRs,genetic diversity,cross-species ampliifcation |
| 主要内容：Simple sequence repeat(SSR) markers were obtained for the large yellow croaker Pseudosciaena crocea using 1205 expressed sequences tags(ESTs) from the NCBI database,Primers for 48 EST-SSR loci were designed and screened with 30 P.crocea specimens captured from Guanjingyang sea area in Fujian Province of China.Sixteen of the loci were polymorphic,which were ampliifed with 3 to 11 alleles per locus and the mean of 6.13.The observed and expected heterozygosity per locus ranged from 0.091 to 0.844(mean 0.544) and from 0.118 to 0.892(mean 0.644),respectively...... |
| 《海洋学报：英文版》 2010(6).-83-91 |
| 全文下载请进入http://hightech.stlib.cn/tpi_1/sysasp/include/index.asp |
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