| Visualization of Golgia apparatus as an intracellular calcium store by laser scanning confocal microscope |
| CUIJIE[1] YANLI[2] 等 |
| 关键词：激光扫描共聚焦显微镜 高尔基体 胞体钙库 细胞 |
| 主要内容：Using laser scanning confocal microscopy,we have found that the in cells loaded with fluo-3/AM,highest intracellular Ca^2+ in the perinuclear region is associated with the Golgi apparatus.The spatiotemporal subcellular distribution of Ca^2+ in living human fibroblasts exposing to calcium-free medium in response to agonists has been investigated.PDGF,which releases Ca^2+ from intracellular stores by inositol(1,4,5)-trisphosphate pathway ,produced a biphasic transient rise in intracellular calcium.The initial rise was resulted from a direct release of calcium from the golgi apparatus.Calcium could be also released from and reaccumulated into the Golgi apparatus by the stimulation of thapsigargin,an inhibitor of the Ca^2+ transport ATPase of intracellular calcium store,Permeablizing the plasma membrane by 10μM digitonin resulted in the calcium release from the Golgi apparatus and depletion of the internal calcium store.These results suggest that the Golgi apparatus plays a role in Ca^2+ regulation in signal transduction. |
| 《细胞研究：英文版》 1995,5(2).-165-179 |
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