| Laser scanning fluorescence microscopic measurement of the movement of cleaving egg surface of Rana Amurensis |
| GUGUOYAN[1] ChengtangXu[2] 等 |
| 关键词：两栖类 蛙卵 卵裂沟 表面活动 新生膜 激光扫描荧光显微测量 |
| 主要内容：By laser scanning fluorescence microscopy for quantitative measurement of fluorescence intensity changes on egg surface stained with fluorescein isothiocyanate during cleavage furrow extending forward,it was found that in area of presumptive cleavage furrow the scanning curve became ∨ shape,indicating dark stripe appeared in that place.Then the fluorescence intensity increased at the place where the bottom of ∨ shape had located,and the scanning curve turned to ∧ shape,indicating single stripe was formed.While enhanced fluorescence appeared on the borders of ∧ shape,an M shape curve was found,showing double stripe occurred.During the distance between two borders of M shape incresing from 50μm to 100μm,a fluorescence peak came to sight in the middle of the M shape,which being the cleavge furrow bottom.The two lateral sides of furrow bottom with decreasing fluorescence were nascent membrane.At that time the curve became W shape.By the sides of cleavage furrow the the stress folds became conspicous after double stripe stage,showing the stretching of the egg surface being increased.With our[31,33]and others[32] reports that polylysine could induce the appearance of nascent membrane and phytohemagglutinins could decrease or prevent the appearance of nascent membrane,we believed the idea of Schroeder[25] that increasing mechanical stress could initiate nascent membrane formation and thought that the stresslay to the outsides of cleavage furrow. |
| 《细胞研究：英文版》 1995,5(1).-9-24 |
| 全文下载请进入http://hightech.stlib.cn/tpi_1/sysasp/include/index.asp |
微信公众号